
During the brief first hearing, Pojamarn pleaded not guilty and asked the court for 90 days in which to prepare documents and witnesses to buttress her defence.
The court agreed and set the date of the next hearing on April 29.
Pojamarn returned to Thailand earlier this month, a trip Thaksin claimed she could reunite with her children.
A court statement also said that Thaksin would return in May to fight the same charges as his wife.
Thaksin and Pojaman are formally charged with abuses of power in the 2003 purchase of the plot of land in central Bangkok from the Financial Institutions Development Fund, a government agency set up to bail out debt-ridden banks.
An anti-corruption law bars prime ministers or their spouses from doing business with government agencies.
Pojamarn also faces charges in connection with a case alleging that she and Thaksin concealed millions of dollarsworth of shares in SC Asset, the family's real estate holding company.